Chromatografie - "barvopis"
Pokud vás ještě neomrzely články věnující se objasňování základních principů chromatografie, tak můžete na internetových stránkách časopisu ABC najít jednoduchý pokus osvětlující základy chromatografie.
Pokus funguje na principu postupu použitého objevitelem chromatografie, ruským botanikem Cvětem, který poprvé použil chromatografii na rozdělení chlorofylových barviv.
Duha z fixů
Ustřihněte obdélníkový proužek filtračního papíru a asi 1,5 cm od dolního okraje udělejte vodorovnou čáru obyčejnou tužkou. Na ní nakreslete vedle sebe krátké čárky jednotlivými barevnými fixy (vyzkoušeno s fixy Centropen, s jinými to může, ale nemusí fungovat podle barviva, které výrobce použil). Tento papír připevněte přehnutím (nebo pomocí izolepy) na kus špejle. Na dno vhodné zavařovačky nalijte 2% vodný roztok kyseliny octové (zřeďte kuchyňský ocet, což je 8% kyselina octová, vodou v poměru 1 díl octa + 3 díly vody).
Poté položte špejli se zavěšeným papírem přes hrdlo sklenice tak, aby dolní okraj papíru (nad kterým jsou nanesené fixové skvrny) byl ponořen do kyseliny octové. Skvrny však musí zůstat nad hladinou! Sklenici zakryjte alobalem, abyste zabránili odparu vody, a počkejte, až hladina rozpouštědla (čelo) nasákne až těsně pod přehnutí, za které je papír zavěšen. Už během toho, jak čelo postupuje, lze pozorovat dělení původně jedné skvrny fixu na několik barevných skvrn. Nejvýše doputují skvrny barviv, která papír zadržuje nejméně, naopak barviva, která drží na papíře velmi silně, zůstanou na místě, kam byla nanesena (na „startu“). Více informací naleznete na stránkách ábíčka.
Pomůcky k pokusu
Filtrační papír (pokud ho neseženete, poslouží i papírový filtr na kávu, piják ze sešitu, výborně funguje i nebarvený toaletní papír), nůžky, tužka (obyčejná, grafitová), barevné fixy Centropen, špejle, izolepa, ocet, zavařovačka, alobal.
««« Předchozí text: Možnosti použití dvourozměrné chromatografie Následující text: Konvenční stacionární fáze »»»
autor: j.u. |
Neděle 13. 04. 2008, 18.25 | tisk | Chromatografie |
14 komentářů | 1124x
Komentáře k textu
Rss komentářů tohoto textu - Formulář pro nový komentář
I had fun reading this post. I want to see more on this subject. Thanks for writing this respectable article. In Any Case, I?m going to sign to your rss and I wish you write great articles again soon.
The secrets of why News is just here.
I will try it this way. There is not much available on this subject. Maybe it's time to expand my horizons. Keep it coming!
where did you pull all this information together? I find it very interesting and was looking for this all daya long.
This is interesting! How did you learn this stuff when you were new to it?
I needed to many thanks for this interesting I positively loved every bit of it. I actually have you bookmarked your web site to envision out the latest stuff you post.
Thank you, a really good read – added to bookmarks so will check back for new content and to read other people's comments. Thanks again.
I certainly got your point of view here. As a matter of fact, If someone would ask my opinion on upgrading, I would definitely say the same.
spending budget bride's savior dream however it may also be considered a nightmare. For anyone who is in search of a fantastic offer in your dream gown, eBay may possibly have just the gown for you personally http://www.waswatches.net . Right here are five guidelines to assist you stay away from disappointment after you are buying for any gown on eBay. one. http://www.waswatches.net/…migiani.html Spend Consideration to Photographs Do not purchase a gown unless of course the vendor has incorporated images on the real gown not only the designer's photographs.A This goes for just about any robe http://www.waswatches.net/…hes-Iwc.html , new or utilized.A Steer clear of listings that only supply the designer image. If this really is a employed gown, you would like to determine images in the gown in it is existing state . If it's a producer who statements they are able to
choosing on the top purse appropriate for the specifications? Right here would be the extremely very best three recommendations that may enable you to are creating the most effective option. If had been asked exactly where inhabits most likely one of the most celebs, the solution will surely be Paris and La. Sure, there is an excessive amount of share of stars; it is going to not be shocking that you simply need to appear throughout Toms Tom Curise http://www.readshoes.com/…lection.html , http://www.readshoes.com Rhianna or somebody else . That's why a good deal of paparazzi are content with their stay appropriate right here. Ashley Greene was noticed by paparazzi acquiring at Gelson's Marketplace location in La. http://www.readshoes.com/…lection.html Getting a pair of flats and pretty informal outfits, she was extremely basic. The sole factor that
I dont recognize how your blog came up, should have been a typo, Your blog appearance good. Have a pleasant day.
are comfy sporting diamonds on their fingers, and 3 stone wedding ceremony rings are fairly the rage there. http://www.isweddingdresses.org p All via this write-up, we have been speaking of on the internet jewellery shops, just mainly because the selection and also the rates you will find unthinkable at your neighborhood jewellery shops. Verify them out, and you are going to concur also.p Concerning the Writer Mel Larson can be a skilled author who enjoys to create about diamond rings, diamond engagement rings, diamond marriage ceremony rings along with the like that had been revealed on weblogs, journals and many publications. http://www.isweddingdresses.org/…es-Veil.html She retains a diploma in Journalism and continues to be within this area for around three many years.p Weddings are notably identified to become among the happiest day`s in an individual existence and but every person cries at them. The thoughts boggles?
stroll in design all yr . Get maintain with the most recent tendencies in women's footwear and most up-to-date promos by signing up using the company's publication.About MakeMeChic:Based mostly within the Metropolis of Market, CA, MakeMeChic is surely an on the web shop that concentrates on stylish women's footwear and attire. MakeMeChic is every single woman's companion on the subject of trendy finds http://www.noonshoes.org . With more than fifty newest designs arriving weekly, a lady will never ever be from design. Press launch created by Cybertegic http://www.noonshoes.org/…omotion.html ?an World-wide-web promoting solutions company that concentrates on research motor optimized press launch advertising and marketing Search engine optimization promoting. .Traverse Town, MI PRWEB. The Snoloha clothes brand name has extra a brand new producers rep group to cover the Eastern Seaboard from South Carolina north towards the New England states, proprietor Rod Contact declared this week.
day energy reserve.You'll discover which the energy reserve show is introduced together with the day show at three o'clock.It moves anticlockwise from one to seven (implying days) displaying the existing power storage.Other functions incorporate the day, month, and also a uncommon four-digit 12 months show.It's the Replicas IWC in-house 51614 calibre that make each one of these features achievable.By way of the clear sapphire case-back, we're permitted to admire the magnificence of your watch's coronary heart, 1 from the greatest automated movements at any time designed.The 62-jewel mechanism operates at a frequency of 21,600 vibrations for each hour and presents a energy reserve of seven days.The h2o resistance of this check out is right down to a hundred meters. Developing legendary luxurious jewellery even iconographic jewellery indicates setting up a signature design.The brand name title may be the 1st and foremost crucial signature of each and every luxurious model.Names like Prada, , Fendi, Versace are family names even for individuals who can't manage to pay for to purchase these manufacturers.All of us know these manufacturers for his or her ultra stylish purses, footwear and clothes.The 2nd most significant signature of the model will be the signature indication including a signature design, pattern, materials, or appear. http://www.sitdresses.org wedding gowns dresses These model signatures occur to eternally symbolize the brand name without having even getting to determine the title from the model.Maybe probably the most signature indicator of any luxurious model belongs to .His eternal monogram canvas using the 3 shapes, normally in brown and mild brown (although not usually, for a multi-colored assortment was introduced), have occur to symbolize the greatest normal luxurious purses.When you have cash, you purchase a monogrammed something and display it off daily.Right here are other signatures of many of the most notable and prestigious jewellery manufacturers. http://www.sitdresses.org formal wedding dresses Italian jewellery designer Roberto Coin's signature can be a concealed ruby or rubellite that touches the pores and skin. http://www.sitdresses.org/…Dresses.html quinceanera Dresses Roberto Coin affectionately refers towards the ruby red signature because the „ruby red spirit“ and ties sporting a ruby to numerous historic cultural beliefs about them which includes that they advertise well being and longevity, fertility and from becoming wounded in battle.A ruby having a gold lining can also be the Roberto Coin brand.Gurhan is famously identified for his historic searching, handmade and hand hammered pure 24 karat gold jewellery with its lustrous gleam and aura.While some jewelers could make some items or collections of hand hammered jewellery, they'll generally use diluted types of gold, for instance
O webu
Náplní těchto stránek je separační metoda zvaná chromatografie.
Slyšíte toto slovo poprvé?
Přečtěte si teoretické základy chromatografie.
Znáte chromatografii?
Pak by vás mohly zajímat novinky, týkající se zejména monolitických stacionárních fází a vícerozměrných separačních technik.
O autorovi
Vědecké zaměřeníProfesní životopis
Seznam publikací
-->