Dvourozměrná kapalinová a plynová chromatografie

Směs syntetických alkylbenzenů byla analyzována pomocí beztrátového online spojení kapilární kapalinové chromatografie (µLC) a kapilární plynové chromatografie (GC), tedy µLC x GC.

Pomocí µLC byla směs rozdělena do strukturních skupin: alifatické uhlovodíky, uhlovodíky s jednou funkční skupinou a uhlovodíky s více funkčními skupinami. Každá frakce z µLC byla poté pomocí speciálního vícesmyčkového převodníku převedena online k analýze pomocí plynové chromatografie.

V průběhu práce byly optimalizovány podmínky separace, a to jak velikost kolony v µLC, tak i rychlost převádění jednotlivých frakcí z převodníku do GC.

Pomocí této techniky je možné získat jak kvantitativní údaje, tak i údaje popisující distribuci množství atomů uhlíku v jednotlivých alkylbenzenech. Opakovatelnost plochy píku, jako koncentrační charakteristiky byla pod 2 % RSD.

J. Chromatogr. A 1181 (2008) 95–102.

««« Předchozí text: Imobilizace enzymů Následující text: Charakterizace porézní struktury monolitů »»»

autor: j.u. | Čtvrtek 31. 01. 2008, 10.18 | tisk | Vícerozměrné techniky | 5 komentářů | 1090x

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Náplní těchto stránek je separační metoda zvaná chromatografie.


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